The Human GPC1 (Glypican-1) CLIA Kit is specifically designed for the accurate detection of glypican-1 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.Glypican-1 is a key protein involved in cell growth, cell adhesion, and signaling pathways, making it a critical factor in various physiological processes.
Dysregulation of glypican-1 has been implicated in diseases such as cancer, diabetes, and neurological disorders, highlighting its importance as a biomarker and potential therapeutic target.With the Human GPC1 CLIA Kit, researchers can accurately measure glypican-1 levels in human samples, allowing for a better understanding of its role in disease pathology and providing valuable insights for the development of novel treatment strategies.
Product Name:
Human GPC1 (Glypican 1) CLIA Kit
SKU:
HUES00955
Target:
Human GPC1 (Glypican 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPC1. You can calculate the concentration of Human GPC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
95-113
91-107
Average (%)
102
103
97
1:4
Range (%)
89-102
100-114
98-114
Average (%)
94
108
104
1:8
Range (%)
100-114
91-106
102-114
Average (%)
106
99
108
1:16
Range (%)
85-97
101-116
85-97
Average (%)
91
108
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
108
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
101-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.84
136.56
397.62
53.19
150.2
386.4
Standard deviation
5.23
11.14
41.83
5.14
12.06
36.75
C V (%)
10.09
8.16
10.52
9.66
8.03
9.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPC1 in samples. No significant cross-reactivity or interference between Human GPC1 and analogues was observed.
