The Human GPX1 (Glutathione Peroxidase 1) ELISA Kit is a powerful tool for accurately measuring GPX1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.GPX1 is a key enzyme in the antioxidant defense system, playing a critical role in protecting cells from oxidative damage. Dysregulation of GPX1 has been linked to various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.
Therefore, measuring GPX1 levels can provide valuable insights into these diseases and contribute to the development of potential therapies.With its advanced technology and proven performance, the Human GPX1 ELISA Kit is an essential tool for researchers studying oxidative stress, antioxidant mechanisms, and related diseases. Trust this kit to deliver accurate and dependable results for your research needs.
Product Name:
Human GPX1 (Glutathione Peroxidase 1) ELISA Kit
SKU:
HUES03414
Target:
Human GPX1 (Glutathione Peroxidase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GPX1. You can calculate the concentration of Human GPX1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
97-114
96-111
Average (%)
102
105
101
1:4
Range (%)
91-106
79-93
88-99
Average (%)
98
85
94
1:8
Range (%)
90-105
85-99
88-98
Average (%)
97
92
93
1:16
Range (%)
87-102
81-92
85-97
Average (%)
94
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.99
204.3
946.17
118.12
214.76
1037.22
Standard deviation
5.67
8.52
51.85
6.08
9.96
44.29
C V (%)
5.11
4.17
5.48
5.15
4.64
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GPX1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPX1 in samples. No significant cross-reactivity or interference between Human GPX1 and analogues was observed.
