The Human GPX1 (Glutathione Peroxidase 1) ELISA Kit is a powerful tool for accurately measuring GPX1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.GPX1 is a key enzyme in the antioxidant defense system, playing a critical role in protecting cells from oxidative damage. Dysregulation of GPX1 has been linked to various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions. Therefore, measuring GPX1 levels can provide valuable insights into these diseases and contribute to the development of potential therapies.With its advanced technology and proven performance, the Human GPX1 ELISA Kit is an essential tool for researchers studying oxidative stress, antioxidant mechanisms, and related diseases. Trust this kit to deliver accurate and dependable results for your research needs.
Product Name:
Human GPX1 (Glutathione Peroxidase 1) ELISA Kit
SKU:
HUES03414
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
