The Human Glutathione Reductase ELISA Kit is a comprehensive solution for detecting and measuring levels of glutathione reductase in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Glutathione reductase is a critical enzyme involved in antioxidant defense mechanisms, playing a key role in maintaining cellular redox balance and protecting cells from oxidative stress.
Dysregulation of glutathione reductase has been linked to various health conditions including oxidative damage, inflammation, and neurodegenerative diseases, highlighting its importance as a biomarker for studying and developing therapies for these conditions.Overall, the Human Glutathione Reductase ELISA Kit provides researchers with a powerful tool to investigate the role of glutathione reductase in human health and disease, offering valuable insights for potential therapeutic interventions.
Product Name:
Human GR (Glutathione Reductase) ELISA Kit
SKU:
HUES02386
Target:
Human GR (Glutathione Reductase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GR. You can calculate the concentration of Human GR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
99-113
93-110
Average (%)
105
105
101
1:4
Range (%)
89-105
87-98
88-103
Average (%)
96
92
95
1:8
Range (%)
88-101
87-98
88-100
Average (%)
95
93
94
1:16
Range (%)
90-102
79-94
83-95
Average (%)
95
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
277.15
530.21
2421.42
287.94
530.38
2592.11
Standard deviation
19.15
31.12
111.14
18.03
30.82
110.68
C V (%)
6.91
5.87
4.59
6.26
5.81
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GR in samples. No significant cross-reactivity or interference between Human GR and analogues was observed.
