The Human GREM1 (Gremlin 1) CLIA Kit is specifically designed for the precise detection of Gremlin 1 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers highly reliable and consistent results, making it an ideal tool for various research applications.Gremlin 1, also known as GREM1, is a key regulator in numerous biological processes including development, tissue homeostasis, and disease progression.
It is closely linked to conditions such as cancer, fibrosis, and developmental disorders, highlighting its importance as a biomarker for studying these diseases and potential therapeutic interventions.With the Human GREM1 CLIA Kit, researchers can uncover valuable insights into the role of Gremlin 1 in various physiological and pathological processes, paving the way for advancements in medical research and treatment strategies.
Product Name:
Human GREM1 (Gremlin 1) CLIA Kit
SKU:
HUES00786
Target:
Human GREM1 (Gremlin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GREM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GREM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GREM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GREM1. You can calculate the concentration of Human GREM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
93-109
87-98
Average (%)
100
101
93
1:4
Range (%)
92-109
90-104
97-108
Average (%)
100
97
102
1:8
Range (%)
97-112
101-115
89-103
Average (%)
104
108
96
1:16
Range (%)
96-112
101-117
101-113
Average (%)
102
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
104
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
100-119
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.99
259.38
913.08
89.99
235.01
883.2
Standard deviation
10.1
23.5
82.45
7.33
26.23
86.29
C V (%)
10.52
9.06
9.03
8.15
11.16
9.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GREM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GREM1 in samples. No significant cross-reactivity or interference between Human GREM1 and analogues was observed.
