Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) CLIA Kit
The Human Gro-alpha (CXCL1) CLIA Kit is a cutting-edge assay designed for the accurate detection of Growth-Regulated Oncogene Alpha levels in human samples. This kit boasts high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.Growth-Regulated Oncogene Alpha, also known as CXCL1, is a potent chemokine involved in inflammatory responses and immune regulation. It plays a key role in diseases such as cancer, inflammation, and immune disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
This innovative kit offers researchers a powerful tool for investigating the role of Growth-Regulated Oncogene Alpha in various biological processes, paving the way for advancements in diagnostic and therapeutic strategies. Unlock the potential of your research with the Human Gro-alpha (CXCL1) CLIA Kit from Assay Genie.
Product Name:
Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) CLIA Kit
SKU:
HUES00044
Target:
Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GROα/CXCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GROα/CXCL1. You can calculate the concentration of Human GROα/CXCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
91-107
88-101
Average (%)
100
99
94
1:4
Range (%)
89-103
83-98
85-98
Average (%)
94
90
90
1:8
Range (%)
103-115
94-111
88-100
Average (%)
109
101
94
1:16
Range (%)
89-102
101-117
97-111
Average (%)
97
107
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
100-116
108
Cell culture media (n=5)
99-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.54
104.64
305.18
35.77
105.44
307.53
Standard deviation
4.18
11.18
34.82
3.19
9.04
29.4
C V (%)
10.85
10.68
11.41
8.92
8.57
9.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GROα/CXCL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GROα/CXCL1 in samples. No significant cross-reactivity or interference between Human GROα/CXCL1 and analogues was observed.
