Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit
The Human Gro-alpha (CXCL1) ELISA Kit is specifically designed for the precise measurement of growth-regulated oncogene alpha levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes. Gro-alpha, also known as CXCL1, is a critical chemokine involved in inflammation and immune response regulation.
Its role in promoting cell proliferation and migration makes it a crucial player in diseases such as cancer, inflammatory disorders, and autoimmune conditions. As a valuable biomarker, the Gro-alpha ELISA Kit provides researchers with a powerful tool for studying these conditions and exploring potential therapeutic interventions.
Product Name:
Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit
SKU:
HUES01332
Target:
Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GROα/CXCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GROα/CXCL1. You can calculate the concentration of Human GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
100-111
92-106
Average (%)
106
105
99
1:4
Range (%)
94-107
89-99
96-108
Average (%)
101
94
102
1:8
Range (%)
93-107
83-96
96-110
Average (%)
101
88
104
1:16
Range (%)
94-107
87-99
99-112
Average (%)
101
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.2
123.4
415.7
58.0
119.7
397.4
Standard deviation
3
6.8
13.7
3.6
6.5
14.7
C V (%)
5.43
5.51
3.3
6.21
5.43
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GROα/CXCL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GROα/CXCL1 in samples. No significant cross-reactivity or interference between Human GROα/CXCL1 and analogues was observed.
