Human GRO beta (Growth Regulated Oncogene Beta) CLIA Kit
The Human Growth-Regulated Oncogene Beta (GRO-β) CLIA Kit is specifically designed for the accurate detection of GRO-β levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.GRO-β, also known as CXCL2, is a protein that plays a crucial role in inflammation and immune response regulation. It is involved in cell growth, differentiation, and migration, making it a valuable biomarker for studying conditions such as cancer, inflammatory diseases, and autoimmunity.
By accurately measuring GRO-β levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human GRO-β CLIA Kit is a valuable tool for researchers looking to investigate the role of GRO-β in various biological processes and disease states. Its high performance and reliability make it an essential addition to any research laboratory aiming to advance our understanding of GRO-β biology.
Product Name:
Human GRO beta (Growth Regulated Oncogene Beta) CLIA Kit
SKU:
HUES01050
Target:
Human GRO beta (Growth Regulated Oncogene Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GROβ/CXCL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GROβ/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROβ/CXCL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GROβ/CXCL2. You can calculate the concentration of Human GROβ/CXCL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
100-113
89-103
Average (%)
100
107
96
1:4
Range (%)
94-111
86-99
91-105
Average (%)
102
93
96
1:8
Range (%)
85-96
103-115
85-96
Average (%)
91
109
90
1:16
Range (%)
92-106
101-118
88-103
Average (%)
98
108
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
90
EDTA plasma (n=5)
87-104
95
Cell culture media (n=5)
95-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.06
51.86
188.79
26.45
54.81
174.47
Standard deviation
2.37
5.27
19.88
2.78
5.24
15.06
C V (%)
9.85
10.16
10.53
10.51
9.56
8.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GROβ/CXCL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Human GROβ/CXCL2 and analogues was observed.
