Human GRO beta (Growth Regulated Oncogene Beta) ELISA Kit
The Human Gro-beta (Growth Regulated Oncogene Beta) ELISA Kit from Assay Genie is specifically designed for the accurate measurement of Gro-beta levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Gro-beta, also known as CXCL2, is a key regulator of inflammation and immune response. It plays a crucial role in mediating the recruitment of neutrophils to sites of infection or injury, as well as promoting tumor growth and metastasis.
Detecting Gro-beta levels can provide valuable insights into the pathogenesis of various diseases, including cancer, inflammatory disorders, and autoimmune conditions.By utilizing the Human Gro-beta ELISA Kit from Assay Genie, researchers can gain a better understanding of Gro-beta's role in health and disease, paving the way for potential therapeutic interventions and improved patient outcomes.
Product Name:
Human GRO beta (Growth Regulated Oncogene Beta) ELISA Kit
SKU:
HUES02851
Target:
Human GRO beta (Growth Regulated Oncogene Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GROβ/CXCL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GROβ/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROβ/CXCL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GROβ/CXCL2. You can calculate the concentration of Human GROβ/CXCL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
93-107
91-105
Average (%)
91
98
96
1:4
Range (%)
100-112
81-93
89-107
Average (%)
106
87
97
1:8
Range (%)
102-117
87-98
99-111
Average (%)
107
92
105
1:16
Range (%)
96-109
88-101
96-107
Average (%)
103
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.7
135.5
382.4
59.7
146.5
360.8
Standard deviation
3.7
7.6
15.3
3.0
6.6
17.7
C V (%)
6.76
5.61
4.0
5.03
4.51
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GROβ/CXCL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Human GROβ/CXCL2 and analogues was observed.
