Human GRP78 (Glucose Regulated Protein 78) ELISA Kit
The Human GRP78 (Glucose-Regulated Protein 78) ELISA Kit is specifically designed for the precise and reliable measurement of GRP78 levels in human biological samples such as serum, plasma, and cell culture supernatants. GRP78, also known as BiP, is a key molecular chaperone involved in protein folding and assembly, particularly in response to cellular stress such as glucose deprivation or endoplasmic reticulum (ER) stress. Abnormal expression of GRP78 has been linked to various diseases including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for research and diagnostic purposes.
This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for studying the role of GRP78 in disease pathogenesis and potential therapeutic interventions. Ideal for a wide range of research applications, the Human GRP78 ELISA Kit provides a valuable tool for investigating the molecular mechanisms underlying various health conditions.
Product Name:
Human GRP78 (Glucose Regulated Protein 78) ELISA Kit
SKU:
HUES03558
Target:
Human GRP78 (Glucose Regulated Protein 78) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GRP78. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRP78 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRP78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GRP78. You can calculate the concentration of Human GRP78 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
95-108
88-101
Average (%)
105
101
94
1:4
Range (%)
89-104
85-99
83-94
Average (%)
97
90
89
1:8
Range (%)
87-102
88-101
87-97
Average (%)
94
93
92
1:16
Range (%)
91-107
83-96
87-100
Average (%)
98
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.99
4.19
19.92
2.04
4.48
18.55
Standard deviation
0.13
0.18
1.1
0.1
0.19
0.64
C V (%)
6.53
4.3
5.52
4.9
4.24
3.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GRP78 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GRP78 in samples. No significant cross-reactivity or interference between Human GRP78 and analogues was observed.
