The Human GUS (Beta-Glucuronidase) Elisa Kit is a powerful tool for researchers looking to accurately measure levels of beta-glucuronidase in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring that results are reliable and reproducible for a variety of research applications.Beta-glucuronidase is an important enzyme involved in the breakdown of complex molecules in the body, playing a key role in drug metabolism and the detoxification process.
Dysregulation of beta-glucuronidase activity has been linked to various diseases and conditions, making it a valuable biomarker for studying and developing potential treatments for these conditions.With its high-quality components and user-friendly protocol, the Human GUS (Beta-Glucuronidase) Elisa Kit from Assay Genie is a valuable tool for researchers investigating the role of beta-glucuronidase in health and disease.
Product Name:
Human GUS beta (beta-glucuronidase) ELISA Kit
SKU:
HUES02113
Target:
Human GUS beta (beta-glucuronidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GUSβ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GUSβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GUSβ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GUSβ. You can calculate the concentration of Human GUSβ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
86-98
95-107
Average (%)
100
91
101
1:4
Range (%)
92-109
82-93
84-97
Average (%)
99
88
89
1:8
Range (%)
91-105
86-99
85-98
Average (%)
99
91
91
1:16
Range (%)
89-101
80-92
81-94
Average (%)
96
85
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.29
4.89
0.52
1.31
5.13
Standard deviation
0.03
0.05
0.25
0.03
0.06
0.17
C V (%)
6.38
3.88
5.11
5.77
4.58
3.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GUSβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GUSβ in samples. No significant cross-reactivity or interference between Human GUSβ and analogues was observed.
