The Human GUS (Beta-Glucuronidase) Elisa Kit is a powerful tool for researchers looking to accurately measure levels of beta-glucuronidase in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring that results are reliable and reproducible for a variety of research applications.Beta-glucuronidase is an important enzyme involved in the breakdown of complex molecules in the body, playing a key role in drug metabolism and the detoxification process. Dysregulation of beta-glucuronidase activity has been linked to various diseases and conditions, making it a valuable biomarker for studying and developing potential treatments for these conditions.With its high-quality components and user-friendly protocol, the Human GUS (Beta-Glucuronidase) Elisa Kit from Assay Genie is a valuable tool for researchers investigating the role of beta-glucuronidase in health and disease.
Product Name:
Human GUS beta (beta-glucuronidase) ELISA Kit
SKU:
HUES02113
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
