The Human GZMB (Granzyme B) ELISA Kit is specifically designed for the precise measurement of granzyme B levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable tool for various research purposes.Granzyme B is a vital enzyme involved in immune responses, particularly cytotoxic T cell-mediated apoptosis.
It plays a crucial role in immune surveillance, defense against infections, and anti-tumor immunity. Monitoring granzyme B levels can provide valuable insights into the immune system function and aid in the development of immunotherapies for cancer and other diseases.Get your Human GZMB ELISA Kit today and unlock the potential for groundbreaking discoveries in immunology and cancer research.
Product Name:
Human GzmB (Granzyme B) ELISA Kit
SKU:
HUES02621
Target:
Human GzmB (Granzyme B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GzmB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GzmB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GzmB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GzmB. You can calculate the concentration of Human GzmB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
98-112
84-96
Average (%)
92
104
90
1:4
Range (%)
89-100
83-97
83-93
Average (%)
95
88
89
1:8
Range (%)
89-103
83-97
80-92
Average (%)
96
90
87
1:16
Range (%)
89-106
80-91
87-101
Average (%)
96
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.88
110.65
427.99
50.22
101.94
413.82
Standard deviation
2.79
5.95
19.94
2.64
4.69
14.19
C V (%)
5.18
5.38
4.66
5.26
4.6
3.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GzmB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GzmB in samples. No significant cross-reactivity or interference between Human GzmB and analogues was observed.
