The Human HAase (Hyaluronidase) ELISA Kit is specifically designed for the precise measurement of hyaluronidase levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research purposes. Hyaluronidase is an enzyme known for its role in breaking down hyaluronic acid, a key component of the extracellular matrix. This enzyme is involved in numerous biological processes such as tissue remodeling, inflammation, and wound healing.
Its dysregulation has been linked to various diseases including cancer, arthritis, and skin disorders, highlighting its importance as a biomarker in studying these conditions and potentially developing therapeutic interventions. The Human HAase ELISA Kit provides researchers with a reliable tool to investigate the levels of hyaluronidase in biological samples, offering valuable insights into its physiological and pathological roles. Don't miss out on the opportunity to enhance your research with this cutting-edge ELISA kit from AssayGenie.
Product Name:
Human HAase (Hyaluronidase) ELISA Kit
SKU:
HUES03096
Target:
Human HAase (Hyaluronidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HAase. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HAase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HAase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HAase. You can calculate the concentration of Human HAase in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
93-107
90-105
Average (%)
98
99
96
1:4
Range (%)
86-99
79-92
84-97
Average (%)
93
85
90
1:8
Range (%)
87-100
79-91
84-98
Average (%)
93
85
91
1:16
Range (%)
87-101
80-89
87-97
Average (%)
94
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.09
31.24
82.97
10.6
32.43
80.64
Standard deviation
0.63
1.8
3.39
0.72
1.89
2.69
C V (%)
6.24
5.76
4.09
6.79
5.83
3.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HAase concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HAase in samples. No significant cross-reactivity or interference between Human HAase and analogues was observed.
