The Human HB (Hemoglobin) CLIA Kit is specifically designed for the quantitative measurement of hemoglobin levels in human serum and plasma samples. This kit offers exceptional sensitivity and accuracy, providing reliable and consistent results for various research applications.Hemoglobin is a crucial protein responsible for transporting oxygen in the blood, and abnormalities in hemoglobin levels can indicate various health conditions such as anemia, polycythemia, or blood disorders.
Monitoring hemoglobin levels is essential for diagnosing and managing these conditions, making this kit a valuable tool for researchers and healthcare professionals.With its easy-to-use format and quick assay time, the Human HB CLIA Kit is an efficient and cost-effective solution for studying hemoglobin levels and related disorders. Order now to advance your research and improve patient care.
Product Name:
Human HB (Hemoglobin) CLIA Kit
SKU:
HUES00297
Target:
Human HB (Hemoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HB. You can calculate the concentration of Human HB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
99-112
95-111
Average (%)
96
106
101
1:4
Range (%)
92-107
89-104
97-110
Average (%)
98
95
103
1:8
Range (%)
87-100
99-115
87-101
Average (%)
94
105
93
1:16
Range (%)
99-112
89-105
95-109
Average (%)
106
96
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.83
6.93
0.87
2.93
6.91
Standard deviation
0.1
0.21
0.49
0.11
0.25
0.57
C V (%)
10.64
7.42
7.07
12.64
8.53
8.25
Sample type &Sample volume:
Serum, plasma, erythrocyte lysates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HB concentrations in Serum, plasma, erythrocyte lysates.
Specificity:
This kit recognizes Human HB in samples. No significant cross-reactivity or interference between Human HB and analogues was observed.
