The Human HB (Hemoglobin) ELISA Kit is specially designed to accurately measure levels of hemoglobin in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it a valuable tool for various research purposes.Hemoglobin is a vital protein responsible for carrying oxygen in the blood, and abnormalities in its levels can indicate various health conditions such as anemia, sickle cell disease, and other blood disorders.
By using this ELISA kit, researchers can gain valuable insights into these conditions and potentially develop new treatment strategies.Overall, the Human HB (Hemoglobin) ELISA Kit is a reliable and efficient tool for studying hemoglobin levels in human samples, offering researchers a comprehensive solution for their investigative needs.
Product Name:
Human HB (Hemoglobin) ELISA Kit
SKU:
HUES01628
Target:
Human HB (Hemoglobin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HB. You can calculate the concentration of Human HB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
95-111
89-104
Average (%)
105
102
96
1:4
Range (%)
102-116
84-98
96-108
Average (%)
107
91
102
1:8
Range (%)
101-115
84-98
92-106
Average (%)
106
91
99
1:16
Range (%)
100-116
81-96
94-108
Average (%)
108
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
92-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.5
14.1
49.3
5.0
12.8
50.1
Standard deviation
0.3
0.6
1.8
0.3
0.5
2.7
C V (%)
5.45
4.26
3.65
6.0
3.91
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HB in samples. No significant cross-reactivity or interference between Human HB and analogues was observed.
