Description
Human HBF (Fetal Hemoglobin) ELISA Kit
The Human HBF (Fetal Hemoglobin) ELISA Kit is a state-of-the-art assay designed for the precise and sensitive quantification of fetal hemoglobin levels in human samples.
Fetal hemoglobin, a unique form of hemoglobin expressed during fetal development, holds paramount importance in oxygen transport and maternity health. Understanding the levels and dynamics of fetal hemoglobin offers insights into various hematological disorders, including sickle cell disease and thalassemia. With exceptional sensitivity and specificity, the Human HBF ELISA Kit enables researchers to accurately measure fetal hemoglobin concentrations, paving the way for comprehensive investigations into hemoglobinopathies and prenatal development. By providing a reliable tool for quantifying fetal hemoglobin, this ELISA kit empowers researchers to explore the role of fetal hemoglobin in health and disease, potentially leading to novel therapeutic strategies and diagnostic approaches. Manufactured under stringent quality control standards, the Human HBF ELISA Kit offers robust performance and reproducible results, ensuring data accuracy and experimental reliability. Researchers investigating fetal hemoglobin biology, hematological disorders, and prenatal diagnostics can benefit from this user-friendly kit to enhance their research efforts and advance our understanding of hemoglobin regulation in human biology.
| Product Name: | Human HBF (Fetal Hemoglobin) ELISA Kit |
| Product Code: | AEES00668 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 12.50-800 ng/mL |
| Sensitivity: | 7.50 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human HBF in samples. No significant cross-reactivity or interference between Human HBF and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human HBF concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HBF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HBF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HBF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HBF. You can calculate the concentration of Human HBF in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
