Description
| Product Name: | Nanodisc Human HCAR2-Strep Protein |
| SKU: | HDFP1052 |
| Size: | 10μg |
| Target: | HCAR2 |
| Tag: | C-Flag&Strep Tag |
| Expression Host: | HEK293 |
| Conjugate: | Unconjugated |
| Synonyms: | GPR109A, HCA2, HM74a, HM74b, NIACR1, PUMAG, Puma-g |
| Protein Families: | GPCR, Transmembrane, Druggable Genome. |
| Protein Description: | Human HCAR2-Strep full length protein-synthetic nanodisc |
| Molecular Weight: | The human full length HCAR2-Strep protein has a MW of 41.9 kDa |
Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.[UniProtKB/Swiss-Prot Function]
| Uniprot ID: | Q8TDS4 |
| Formulation & Reconstitution: | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping: | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Usage: | Research use only |
