The Human HE4 (epididymal protein 4) ELISA Kit is a specialized assay intended for the precise quantification of HE4 levels in human biological samples. HE4, also known as a tumor marker, is a glycoprotein that is involved in various biological functions and is predominantly expressed in the male reproductive tract and female genital organs. Elevated levels of HE4 have been linked to gynecologic cancers, making it a valuable biomarker for the early detection and monitoring of ovarian and endometrial cancers. This ELISA kit offers a reliable platform for researchers and clinicians to accurately measure HE4 levels in serum or plasma samples, facilitating investigations related to cancer diagnostics, prognosis, and therapeutic monitoring.
By detecting changes in HE4 expression, researchers can gain insights into disease progression, response to treatment, and overall patient management. The Human HE4 ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and ease of use, making it an excellent choice for research studies focusing on cancer biomarkers, tumor detection, and personalized medicine approaches.
Product Name:
Human HE4 (epididymal protein 4) ELISA Kit
SKU:
AEES00656
Target:
Human HE4 (epididymal protein 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
14.13 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HE4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HE4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HE4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HE4. You can calculate the concentration of Human HE4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
101-113
89-101
Average (%)
98
106
96
1:4
Range (%)
92-106
100-114
90-103
Average (%)
97
107
97
1:8
Range (%)
88-102
96-107
90-106
Average (%)
95
102
97
1:16
Range (%)
94-110
99-116
90-101
Average (%)
101
106
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
126.5
380.77
1247.35
128.94
390.9
1196.14
Standard deviation
8.41
17.52
67.23
7.56
17.12
64.11
C V (%)
6.65
4.6
5.39
5.86
4.38
5.36
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HE4 concentrations in .
Specificity:
This kit recognizes Human HE4 in samples. No significant cross-reactivity or interference between Human HE4 and analogues was observed.
