Human Heat Shock 70kDa Binding Protein 1 (HSPBP1) ELISA Kit
The Human Heat Shock 70kDa Binding Protein 1 (HSPBP1) ELISA Kit is a powerful tool for the precise measurement of HSPBP1 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for a wide range of research applications.HSPBP1 is a critical protein that interacts with heat shock proteins and plays a key role in cellular stress response and protein folding. Dysregulation of HSPBP1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions, highlighting its importance as a potential therapeutic target and diagnostic marker.
By utilizing the Human HSPBP1 ELISA Kit, researchers can gain valuable insights into the role of HSPBP1 in disease pathogenesis and progression, paving the way for the development of novel treatment strategies. Don't miss out on this essential tool for studying HSPBP1 biology and its implications in human health.
Product Name:
Human Heat Shock 70kDa Binding Protein 1 (HSPBP1) ELISA Kit
Product Code:
HUDL01396
Size:
96 Assays
Target:
HSPBP1
Synonyms:
FES1, Hsp70 Interacting Protein, Hsp70 Binding Protein,Cytoplasmic Cochaperone 1, Heat shock protein-binding protein 1, Hsp70-interacting protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
