The Human HO-1 (Heme Oxygenase-1) CLIA Kit is a powerful tool for the precise quantification of HO-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results, making it ideal for a wide range of research applications.HO-1 is a vital enzyme involved in the breakdown of heme, playing a key role in cellular defense against oxidative stress and inflammation. Dysregulation of HO-1 has been implicated in various diseases including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
Overall, the Human HO-1 CLIA Kit provides researchers with a reliable and efficient platform for investigating the role of HO-1 in human health and disease, offering valuable insights for furthering our understanding of these complex conditions.
Product Name:
Human HO1 (Heme Oxygenase 1) CLIA Kit
SKU:
HUES01148
Target:
Human HO1 (Heme Oxygenase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HO1. You can calculate the concentration of Human HO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
86-101
96-108
Average (%)
94
93
102
1:4
Range (%)
89-101
99-116
85-98
Average (%)
96
107
91
1:8
Range (%)
97-114
95-109
91-107
Average (%)
104
100
98
1:16
Range (%)
93-106
86-102
96-111
Average (%)
99
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.38
577.95
1706.82
231.85
593.77
1749.08
Standard deviation
19.64
49.99
176.31
20.01
52.43
136.78
C V (%)
8.91
8.65
10.33
8.63
8.83
7.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HO1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HO1 in samples. No significant cross-reactivity or interference between Human HO1 and analogues was observed.
