Human HSF1 (Heat Shock Transcription Factor 1) CLIA Kit
The Human HSF1 (Heat Shock Transcription Factor 1) CLIA Kit is designed for the accurate detection of HSF1 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.HSF1 is a key transcription factor that regulates the expression of heat shock proteins in response to cellular stress. It plays a crucial role in maintaining cellular homeostasis and protecting cells from damage.
Dysregulation of HSF1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it an important biomarker for studying these conditions and developing potential therapies.Overall, the Human HSF1 CLIA Kit provides researchers with a valuable tool for accurately measuring HSF1 levels and investigating its role in health and disease.
Product Name:
Human HSF1 (Heat Shock Transcription Factor 1) CLIA Kit
SKU:
HUES01033
Target:
Human HSF1 (Heat Shock Transcription Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSF1. You can calculate the concentration of Human HSF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
88-101
94-106
Average (%)
102
94
100
1:4
Range (%)
85-97
88-101
99-113
Average (%)
92
94
107
1:8
Range (%)
84-99
91-105
87-98
Average (%)
91
98
93
1:16
Range (%)
90-102
96-111
88-98
Average (%)
95
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
98-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
201.95
621.84
1686.34
199.52
625.53
1839.13
Standard deviation
16.46
67.41
128.16
24.86
56.8
111.45
C V (%)
8.15
10.84
7.6
12.46
9.08
6.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSF1 in samples. No significant cross-reactivity or interference between Human HSF1 and analogues was observed.
