Human HSF2 (Heat Shock Transcription Factor 2) CLIA Kit
The Human HSF2 (Heat Shock Transcription Factor 2) CLIA Kit is a precise and reliable tool for detecting HSF2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.HSF2 is a critical transcription factor involved in the cellular response to stress, including heat shock and other environmental stimuli. Its role in regulating the expression of heat shock proteins makes it a key player in maintaining cellular homeostasis and protecting against cellular damage.
Dysregulation of HSF2 has been linked to various diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human HSF2 CLIA Kit provides researchers with a powerful tool for investigating the role of HSF2 in health and disease, offering insights that can drive advancements in personalized medicine and targeted therapies.
Product Name:
Human HSF2 (Heat Shock Transcription Factor 2) CLIA Kit
SKU:
HUES01032
Target:
Human HSF2 (Heat Shock Transcription Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSF2. You can calculate the concentration of Human HSF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
83-98
89-104
Average (%)
105
90
95
1:4
Range (%)
96-107
84-98
88-104
Average (%)
101
90
95
1:8
Range (%)
85-96
100-118
85-97
Average (%)
91
107
91
1:16
Range (%)
94-106
102-116
87-103
Average (%)
99
107
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.38
179.86
719.91
93.41
167.67
654.71
Standard deviation
12.16
20.9
48.38
8.9
13.58
41.31
C V (%)
12.11
11.62
6.72
9.53
8.1
6.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSF2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSF2 in samples. No significant cross-reactivity or interference between Human HSF2 and analogues was observed.
