The Human HSP-90 (Heat Shock Protein 90) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of HSP-90 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for a variety of research applications.HSP-90 is a molecular chaperone protein that plays a critical role in protein folding, stability, and regulation of client proteins. It is involved in various cellular processes and is often upregulated in response to cellular stress, making it a key player in maintaining cellular homeostasis.
Studying HSP-90 levels can provide valuable insights into cellular stress responses, protein quality control mechanisms, and the development of therapeutic strategies for diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. The Human HSP-90 CLIA Kit is an invaluable tool for researchers looking to investigate the role of HSP-90 in health and disease.
Product Name:
Human HSP-90 (Heat Shock Protein 90) CLIA Kit
SKU:
HUES01031
Target:
Human HSP-90 (Heat Shock Protein 90)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-90. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSP-90. You can calculate the concentration of Human HSP-90 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
86-98
94-109
Average (%)
104
92
100
1:4
Range (%)
99-111
99-115
99-114
Average (%)
106
105
105
1:8
Range (%)
96-107
101-117
100-119
Average (%)
101
109
108
1:16
Range (%)
96-111
83-99
90-106
Average (%)
102
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
100-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
222.05
377.85
1698.93
237.82
375.25
1576.39
Standard deviation
23.32
35.33
156.3
22.14
43.42
166.15
C V (%)
10.5
9.35
9.2
9.31
11.57
10.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSP-90 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-90 in samples. No significant cross-reactivity or interference between Human HSP-90 and analogues was observed.
