Human HSP beta6/HSP-20 (Heat Shock Protein Beta 6) CLIA Kit
The Human HSP Beta-6 (HSP-20, Heat Shock Protein Beta-6) CLIA Kit is specifically designed for the precise quantification of HSP Beta-6 levels in human biological samples such as serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.HSP Beta-6, also known as HSP-20, is a key member of the heat shock protein family that plays a critical role in cellular stress response and protein folding. Dysregulation of HSP Beta-6 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions, highlighting its importance as a potential therapeutic target and biomarker.
By using the Human HSP Beta-6 CLIA Kit, researchers can gain valuable insights into the role of HSP Beta-6 in disease pathogenesis, as well as explore its potential as a diagnostic marker or therapeutic target. Trust in the accuracy and reliability of this kit to advance your research efforts and uncover novel discoveries in the field of heat shock protein biology.
Product Name:
Human HSP beta6/HSP-20 (Heat Shock Protein Beta 6) CLIA Kit
SKU:
HUES01026
Target:
Human HSP beta6/HSP-20 (Heat Shock Protein Beta 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSPβ6/HSP-20. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSPβ6/HSP-20 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSPβ6/HSP-20, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSPβ6/HSP-20. You can calculate the concentration of Human HSPβ6/HSP-20 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
100-113
85-99
Average (%)
92
105
91
1:4
Range (%)
89-100
89-104
97-111
Average (%)
94
96
103
1:8
Range (%)
99-115
95-111
90-107
Average (%)
106
103
98
1:16
Range (%)
99-112
89-101
102-119
Average (%)
105
95
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
95-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.05
534.82
1755.1
215.39
560.48
1872.07
Standard deviation
20.38
53.96
157.43
24.81
42.99
121.68
C V (%)
9.22
10.09
8.97
11.52
7.67
6.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSPβ6/HSP-20 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSPβ6/HSP-20 in samples. No significant cross-reactivity or interference between Human HSPβ6/HSP-20 and analogues was observed.
