Human Hyaluronan Binding Protein 2 (HABP2) ELISA Kit
The Human Hyaluronan Binding Protein 2 (HABP2) ELISA Kit is a powerful tool for measuring levels of HABP2 in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and dependable results for various research applications. HABP2 is a key protein involved in hyaluronan binding, playing a critical role in cell adhesion and migration. Dysregulation of HABP2 has been linked to various diseases, including cancer, inflammation, and fibrosis, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
By using the Human HABP2 ELISA Kit, researchers can gain valuable insights into HABP2 levels in human samples, advancing our understanding of its role in disease development and progression. Trust in this kit for robust and precise measurement of HABP2 levels, enabling impactful research discoveries.
Product Name:
Human Hyaluronan Binding Protein 2 (HABP2) ELISA Kit
Product Code:
HUDL01297
Size:
96 Assays
Target:
HABP2
Synonyms:
FSAP, HABP, HGFAL, PHBP, Plasma Hyaluronan Binding Protein, Factor VII Activating Protein, Factor seven-activating protease, Hepatocyte growth factor activator-like protein
Detection Method:
Sandwich
Range:
78.125-5,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
