The Human HYOU1 (Hypoxia Up-regulated 1) CLIA Kit is specially designed for the precise measurement of HYOU1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.HYOU1 is a key protein that is upregulated in response to hypoxic conditions, playing a crucial role in cell survival and adaptation to low oxygen levels. Its involvement in various diseases, including cancer, ischemic disorders, and inflammatory conditions, makes it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
With its high-quality components and user-friendly protocol, the Human HYOU1 CLIA Kit is an essential tool for researchers aiming to investigate the role of HYOU1 in disease pathogenesis and therapeutic development. Trust this kit to deliver reliable and insightful data for your scientific endeavors.
Product Name:
Human HYOU1 (Hypoxia Up Regulated 1) CLIA Kit
SKU:
HUES00308
Target:
Human HYOU1 (Hypoxia Up Regulated 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HYOU1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HYOU1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HYOU1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HYOU1. You can calculate the concentration of Human HYOU1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
103-120
88-103
Average (%)
105
110
94
1:4
Range (%)
96-114
88-102
96-111
Average (%)
104
94
103
1:8
Range (%)
86-101
87-102
100-113
Average (%)
93
93
106
1:16
Range (%)
90-104
95-110
88-101
Average (%)
97
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
98-112
104
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.55
208.06
994.36
91.87
214.6
923.19
Standard deviation
11.22
17.81
111.67
9.52
24.68
92.78
C V (%)
12.12
8.56
11.23
10.36
11.5
10.05
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HYOU1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human HYOU1 in samples. No significant cross-reactivity or interference between Human HYOU1 and analogues was observed.
