Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) CLIA Kit
The Human I-TAC (Interferon-Inducible T Cell Alpha Chemoattractant) CLIA Kit is a reliable and accurate tool for detecting levels of this important chemokine in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a variety of research applications.I-TAC is a key chemokine involved in the recruitment and activation of T cells, playing a crucial role in the immune response to various diseases and infections.
Its measurement can provide valuable insights into immune system function and inflammatory processes, making it a valuable biomarker for studying conditions such as autoimmune disorders, infectious diseases, and cancer.Overall, the Human I-TAC CLIA Kit offers researchers a powerful tool for studying the role of this important chemokine in health and disease, helping to advance our understanding of immune responses and potentially identify new therapeutic targets.
Product Name:
Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) CLIA Kit
SKU:
HUES00049
Target:
Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human I-TAC/CXCL11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human I-TAC/CXCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human I-TAC/CXCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human I-TAC/CXCL11. You can calculate the concentration of Human I-TAC/CXCL11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
87-98
86-103
Average (%)
105
92
94
1:4
Range (%)
90-103
93-107
91-102
Average (%)
95
98
96
1:8
Range (%)
99-116
96-113
87-99
Average (%)
106
103
92
1:16
Range (%)
87-99
95-111
101-114
Average (%)
93
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
105
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
96-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.41
115.32
466.51
55.92
108.7
461.95
Standard deviation
6.22
12.56
48.24
5.7
8.19
29.93
C V (%)
12.1
10.89
10.34
10.19
7.53
6.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human I-TAC/CXCL11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human I-TAC/CXCL11 in samples. No significant cross-reactivity or interference between Human I-TAC/CXCL11 and analogues was observed.
