Human ICAM4 (Intercellular Adhesion Molecule 4) CLIA Kit
The Human ICAM-4 (Intercellular Adhesion Molecule 4) CLIA Kit is a highly sensitive and reliable assay designed for the precise detection of ICAM-4 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for a wide range of research applications.ICAM-4 is a cell adhesion molecule that plays a crucial role in mediating cell-cell interactions and is involved in various physiological processes, including immune response and inflammation. Dysregulation of ICAM-4 has been linked to diseases such as autoimmune disorders, inflammatory conditions, and infectious diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.
The Human ICAM-4 CLIA Kit from Assay Genie offers researchers a powerful tool for studying ICAM-4 biology and its implications in disease pathology. With its high sensitivity and specificity, this kit provides accurate quantification of ICAM-4 levels, enabling researchers to gain valuable insights into the role of ICAM-4 in health and disease.
Product Name:
Human ICAM4 (Intercellular Adhesion Molecule 4) CLIA Kit
SKU:
HUES00309
Target:
Human ICAM4 (Intercellular Adhesion Molecule 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ICAM4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICAM4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ICAM4. You can calculate the concentration of Human ICAM4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
85-99
91-105
Average (%)
99
91
98
1:4
Range (%)
101-115
91-104
96-109
Average (%)
107
98
102
1:8
Range (%)
90-101
101-117
98-112
Average (%)
96
109
104
1:16
Range (%)
93-108
92-104
100-119
Average (%)
99
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.04
3.79
18.22
2.17
3.71
17.7
Standard deviation
0.26
0.28
1.86
0.18
0.41
1.79
C V (%)
12.75
7.39
10.21
8.29
11.05
10.11
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ICAM4 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human ICAM4 in samples. No significant cross-reactivity or interference between Human ICAM4 and analogues was observed.
