The Human IDE (Insulin-Degrading Enzyme) CLIA Kit is designed for the precise measurement of IDE levels in human serum, plasma, and cell culture samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.IDE is a key enzyme involved in the breakdown of insulin and amyloid beta, making it a crucial player in maintaining glucose homeostasis and potentially impacting neurodegenerative diseases such as Alzheimer's.
By accurately measuring IDE levels, researchers can better understand its role in disease pathology and potentially identify new therapeutic targets.Overall, the Human IDE CLIA Kit is a valuable tool for studying the role of IDE in various diseases and exploring potential treatment options. Its reliable performance and ease of use make it a valuable asset for any laboratory conducting research in the field of insulin degradation and related disorders.
Product Name:
Human IDE (Insulin Degrading Enzyme) CLIA Kit
SKU:
HUES00310
Target:
Human IDE (Insulin Degrading Enzyme)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IDE. You can calculate the concentration of Human IDE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
94-108
86-97
Average (%)
98
99
91
1:4
Range (%)
101-116
87-101
103-117
Average (%)
107
93
109
1:8
Range (%)
87-101
98-111
91-103
Average (%)
92
104
96
1:16
Range (%)
88-99
96-113
103-119
Average (%)
94
103
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
98-113
104
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.47
111.52
391.0
48.69
108.49
395.33
Standard deviation
6.33
9.32
25.42
4.27
10.66
43.37
C V (%)
12.8
8.36
6.5
8.77
9.83
10.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IDE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IDE in samples. No significant cross-reactivity or interference between Human IDE and analogues was observed.
