The Human IgA (Immunoglobulin A) CLIA Kit is a reliable and accurate tool for measuring IgA levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring consistent and precise results for a variety of research applications.Immunoglobulin A is an important antibody that plays a significant role in the body's immune defense system, particularly in mucosal immunity. Abnormal levels of IgA have been linked to various autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With its user-friendly design and efficient performance, the Human IgA CLIA Kit is an essential tool for researchers and healthcare professionals looking to accurately measure and monitor IgA levels in clinical and research settings. Visit the provided URL for more information and to order your kit today.
Product Name:
Human IgA (Immumoglobulin A) CLIA Kit
SKU:
HUES00789
Target:
Human IgA (Immumoglobulin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgA. You can calculate the concentration of Human IgA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
91-107
85-96
Average (%)
97
98
91
1:4
Range (%)
84-97
102-115
99-111
Average (%)
91
109
105
1:8
Range (%)
95-109
89-105
87-97
Average (%)
103
96
92
1:16
Range (%)
87-101
103-119
89-105
Average (%)
92
110
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.12
19.48
72.22
10.38
19.63
70.31
Standard deviation
1.01
2.21
6.46
1.18
1.54
7.47
C V (%)
9.98
11.34
8.94
11.37
7.85
10.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgA in samples. No significant cross-reactivity or interference between Human IgA and analogues was observed.
