The Human IgE (Immunoglobulin E) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of IgE levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers accurate and reliable results, making it an invaluable asset for various research applications.IgE is a key immunoglobulin involved in allergic reactions and plays a crucial role in the body's immune response to allergens. Elevated IgE levels are associated with allergies, asthma, and other immune-related disorders, making it a vital biomarker for studying these conditions and developing targeted treatments.
This state-of-the-art CLIA kit allows researchers and healthcare professionals to accurately quantify IgE levels, providing valuable insights into the immune response and facilitating personalized treatment strategies for patients with allergic conditions. Partner with Assay Genie today and unlock the potential of IgE biomarker research with the Human IgE CLIA Kit.
Product Name:
Human IgE (Immunoglobulin E) CLIA Kit
SKU:
HUES01142
Target:
Human IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
104-120
101-118
Average (%)
95
110
109
1:4
Range (%)
89-101
85-97
101-117
Average (%)
95
90
107
1:8
Range (%)
95-106
96-111
88-100
Average (%)
100
102
93
1:16
Range (%)
90-107
97-114
86-100
Average (%)
98
104
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
106
EDTA plasma (n=5)
95-111
103
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.99
3.77
16.29
2.1
3.42
17.03
Standard deviation
0.25
0.39
1.3
0.25
0.37
1.14
C V (%)
12.56
10.34
7.98
11.9
10.82
6.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgE in samples. No significant cross-reactivity or interference between Human IgE and analogues was observed.
