Human IGF-2 (Insulin Like Growth Factor 2) CLIA Kit
The Human IGF-2 (Insulin-like Growth Factor 2) CLIA Kit is specifically designed for the accurate and reliable measurement of IGF-2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for precise and reproducible results that are essential for a wide range of research applications.Insulin-like Growth Factor 2 (IGF-2) is a key growth factor that plays a crucial role in cell growth, differentiation, and development. Its dysregulation has been associated with various diseases including cancer, metabolic disorders, and developmental abnormalities.
Therefore, the accurate measurement of IGF-2 levels is vital for understanding its role in disease pathogenesis and for developing targeted therapies.Overall, the Human IGF-2 CLIA Kit is an indispensable tool for researchers studying IGF-2 biology and its implications in various diseases. Its high performance and ease of use make it a valuable asset for any laboratory conducting research in the field of growth factor signaling and disease mechanisms.
Product Name:
Human IGF-2 (Insulin Like Growth Factor 2) CLIA Kit
SKU:
HUES00314
Target:
Human IGF-2 (Insulin Like Growth Factor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGF-2. You can calculate the concentration of Human IGF-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
97-110
99-114
Average (%)
93
103
106
1:4
Range (%)
96-113
85-99
99-113
Average (%)
103
92
104
1:8
Range (%)
86-98
86-99
86-96
Average (%)
93
91
91
1:16
Range (%)
94-109
89-102
100-114
Average (%)
102
96
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
97-112
105
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.99
352.86
1864.55
226.72
375.08
1978.28
Standard deviation
17.91
26.29
200.63
21.72
39.42
152.92
C V (%)
8.14
7.45
10.76
9.58
10.51
7.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF-2 in samples. No significant cross-reactivity or interference between Human IGF-2 and analogues was observed.
