Human IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
The Human IGF-2 (Insulin-Like Growth Factor 2) ELISA Kit is meticulously crafted for the precise quantitative detection of IGF-2 levels in various human biological samples.
IGF-2, a potent growth factor, is actively involved in regulating cell growth, proliferation, and differentiation. It plays a crucial role in various biological processes, including metabolic functions, tissue development, and cellular repair mechanisms, making it an essential component of human growth and development. Accurate quantification of IGF-2 is fundamental in elucidating its role in growth regulation, metabolic pathways, and disease progression. This ELISA kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures, this kit guarantees robust performance, facilitating ease of use in both research and clinical settings. Rely on Assay Genie's IGF-2 ELISA Kit for precise and reliable quantification of this vital growth factor in your research endeavors.
Product Name:
Human IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
SKU:
AEES00073
Target:
Human IGF-2 (Insulin Like Growth Factor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF-2. You can calculate the concentration of Human IGF-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-97
91-104
97-105
Average (%)
92
97
100
1:4
Range (%)
91-104
91-102
92-99
Average (%)
99
98
95
1:8
Range (%)
97-113
87-101
86-100
Average (%)
105
94
94
1:16
Range (%)
90-101
93-101
101-111
Average (%)
94
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
103
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.55
59.05
248.23
24.78
61.03
240.47
Standard deviation
1.1
2.8
11.62
1.29
3.03
13.18
C V (%)
5.1
4.74
4.68
5.21
4.96
5.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF-2 in samples. No significant cross-reactivity or interference between Human IGF-2 and analogues was observed.
