Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) CLIA Kit
The Human IGFBP-5 (Insulin-Like Growth Factor Binding Protein-5) CLIA Kit is a powerful tool for accurately measuring levels of IGFBP-5 in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.IGFBP-5 is a key protein that regulates the actions of insulin-like growth factors, playing a critical role in cell growth, differentiation, and survival. Abnormal levels of IGFBP-5 have been associated with various diseases, including cancer, diabetes, and osteoporosis, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Human IGFBP-5 CLIA Kit offers researchers a reliable and efficient solution for studying the role of IGFBP-5 in various biological processes and disease states. With its high-performance capabilities, this kit is a valuable addition to any laboratory looking to advance their research in the field of growth factor regulation.
Product Name:
Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) CLIA Kit
SKU:
HUES00320
Target:
Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFBP-5. You can calculate the concentration of Human IGFBP-5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
83-95
96-112
Average (%)
95
90
104
1:4
Range (%)
85-98
101-115
94-106
Average (%)
91
108
101
1:8
Range (%)
91-102
98-113
93-105
Average (%)
97
104
99
1:16
Range (%)
99-113
94-109
102-114
Average (%)
105
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.46
8.21
1.17
2.33
8.83
Standard deviation
0.12
0.29
0.81
0.15
0.23
1.01
C V (%)
11.21
11.79
9.87
12.82
9.87
11.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFBP-5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-5 in samples. No significant cross-reactivity or interference between Human IGFBP-5 and analogues was observed.
