The Human IGG2 (Immunoglobulin G2) CLIA Kit is specially designed for the accurate quantification of human IGG2 levels in serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for a wide range of research applications.Immunoglobulin G2 is an important antibody that plays a crucial role in the immune response, particularly in bacterial and viral infections.
Monitoring levels of IGG2 can provide valuable insights into the immune status of individuals and aid in the diagnosis and monitoring of immune-related disorders.This CLIA kit offers convenience and efficiency, allowing for easy and rapid detection of IGG2 levels in biological samples. With its reliable performance, researchers can confidently use this kit to advance their studies in immunology, infectious diseases, and other related fields.
Product Name:
Human IgG2 (Immunoglobulin G2) CLIA Kit
SKU:
HUES00973
Target:
Human IgG2 (Immunoglobulin G2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 ng/mL
Detection range:
93.75-6000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgG2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgG2. You can calculate the concentration of Human IgG2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
85-97
97-109
Average (%)
101
92
103
1:4
Range (%)
88-101
100-116
93-108
Average (%)
94
107
101
1:8
Range (%)
93-103
88-103
95-110
Average (%)
98
94
100
1:16
Range (%)
97-113
102-117
87-100
Average (%)
105
108
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
102
EDTA plasma (n=5)
98-110
103
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
273.5
923.36
2626.87
267.85
939.04
2622.31
Standard deviation
28.53
82.64
196.75
32.36
71.18
181.73
C V (%)
10.43
8.95
7.49
12.08
7.58
6.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgG2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgG2 in samples. No significant cross-reactivity or interference between Human IgG2 and analogues was observed.
