The Human IgM (Immunoglobulin M) CLIA Kit is specifically designed for the sensitive and accurate measurement of IgM levels in human serum, plasma, and other biological samples. Our CLIA kit uses a chemiluminescent immunoassay technology, providing high sensitivity and a wide dynamic range for reliable results.IgM is the largest antibody molecule and is the first antibody produced during an immune response. Elevated levels of IgM can indicate acute infection or autoimmune diseases, making it a valuable biomarker for research and diagnostic purposes.
This kit is easy to use, with a simple protocol and short assay time, making it suitable for high-throughput studies. Whether you are studying infectious diseases, immune disorders, or monitoring patient response to therapy, the Human IgM CLIA Kit from AssayGenie is an essential tool for your research needs. Order now and discover the potential of IgM in your study.
Product Name:
Human IgM (Immunoglobulin M) CLIA Kit
SKU:
HUES01004
Target:
Human IgM (Immunoglobulin M)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgM. You can calculate the concentration of Human IgM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
100-118
91-104
Average (%)
105
107
97
1:4
Range (%)
97-111
86-98
92-105
Average (%)
103
92
98
1:8
Range (%)
95-109
89-103
88-101
Average (%)
101
94
95
1:16
Range (%)
94-110
101-117
101-118
Average (%)
100
107
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.88
29.94
71.54
10.68
27.12
65.16
Standard deviation
1.06
3.41
6.65
0.93
2.78
6.56
C V (%)
9.74
11.39
9.3
8.71
10.25
10.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgM in samples. No significant cross-reactivity or interference between Human IgM and analogues was observed.
