Human IkB alpha (Inhibitory Subunit of NF Kappa B Alpha) CLIA Kit
The Human IKB-Alpha (Inhibitory Subunit of NF-Kappa B Alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of IKB-Alpha levels in human serum, plasma, and cell culture supernatants. This kit provides researchers with a reliable and reproducible tool for studying the role of IKB-Alpha in various cellular processes.IKB-Alpha is a key regulatory protein that plays a crucial role in the NF-Kappa B signaling pathway, which is involved in the regulation of immune responses, inflammation, and cell survival. Dysregulation of this pathway has been linked to various diseases, including cancer, inflammatory disorders, and autoimmune diseases.
Therefore, accurate measurement of IKB-Alpha levels using this CLIA kit is essential for understanding the pathogenesis of these conditions and developing targeted therapies.With its high sensitivity and specificity, the Human IKB-Alpha CLIA Kit from Assay Genie is a valuable tool for researchers looking to advance their understanding of NF-Kappa B signaling and its implications in health and disease. By providing precise measurements of IKB-Alpha levels, this kit enables researchers to make informed decisions and accelerate progress in the field of molecular biology and clinical research.
Product Name:
Human IkB alpha (Inhibitory Subunit of NF Kappa B Alpha) CLIA Kit
SKU:
HUES01254
Target:
Human IkB alpha (Inhibitory Subunit of NF Kappa B Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IκBα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IκBα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IκBα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IκBα. You can calculate the concentration of Human IκBα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
85-98
86-100
Average (%)
94
91
93
1:4
Range (%)
99-110
95-109
94-110
Average (%)
104
102
101
1:8
Range (%)
101-117
93-103
91-103
Average (%)
110
98
96
1:16
Range (%)
103-116
95-106
97-111
Average (%)
109
101
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
93-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.3
211.38
890.41
107.12
197.03
965.95
Standard deviation
9.02
14.97
63.49
8.75
21.14
80.85
C V (%)
9.18
7.08
7.13
8.17
10.73
8.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IκBα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IκBα in samples. No significant cross-reactivity or interference between Human IκBα and analogues was observed.
