The Human IL-6 ELISA Kit is meticulously developed for the quantitative assessment of Interleukin-6 (IL-6) levels in a diverse range of biological samples.
IL-6 is a vital pro-inflammatory cytokine involved in numerous cellular functions, including immune modulation, hematopoiesis, and acute-phase responses. Its role in mediating inflammatory processes and immunity makes accurate IL-6 measurement crucial for understanding immune regulation, inflammatory diseases, and the development of effective therapeutic strategies. Our IL-6 ELISA Kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs. With stringent quality control measures, this kit guarantees robust performance and ease of use, making it an optimal choice for high-quality research applications. Trust Assay Genie's IL-6 ELISA Kit for accurate and dependable quantification of this pivotal cytokine in your studies.
Product Name:
Human IL-6 ELISA Kit
SKU:
AEES00163
Target:
Human IL-6
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
95-106
90-104
Average (%)
95
100
95
1:4
Range (%)
84-96
96-108
86-101
Average (%)
90
101
93
1:8
Range (%)
87-98
89-105
87-100
Average (%)
93
96
93
1:16
Range (%)
85-101
88-102
89-103
Average (%)
92
96
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
93-103
98
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.98
9.9
47.86
5.13
8.95
45.89
Standard deviation
0.29
0.44
2.32
0.33
0.44
1.4
C V (%)
5.78
4.46
4.85
6.49
4.91
3.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.
