The Human IL-18 ELISA Kit is a highly sensitive and specific assay designed for the precise quantification of IL-18 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for researchers in various fields.IL-18, also known as Interleukin-18, is a proinflammatory cytokine involved in immune responses and inflammatory processes. It has been implicated in various diseases such as autoimmune disorders, infectious diseases, and inflammatory conditions, making it a valuable biomarker for studying these pathologies and potential therapeutic targets.
With its reliable performance and ease of use, the Human IL-18 ELISA Kit from Assaygenie is an essential tool for researchers looking to study the role of IL-18 in health and disease. Visit the link provided for more information on this cutting-edge research tool.
Product Name:
Human IL-18 ELISA Kit
SKU:
HUES01492
Target:
Human IL-18
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-18. You can calculate the concentration of Human IL-18 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
96-109
95-112
Average (%)
99
102
102
1:4
Range (%)
97-112
86-101
92-105
Average (%)
103
92
98
1:8
Range (%)
94-108
86-97
94-111
Average (%)
100
91
101
1:16
Range (%)
96-110
81-91
92-107
Average (%)
102
86
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.6
160.5
353.5
56.7
176.1
373.5
Standard deviation
3.3
7.5
17.0
3.9
7.7
13.4
C V (%)
6.04
4.67
4.81
6.88
4.37
3.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-18 in samples. No significant cross-reactivity or interference between Human IL-18 and analogues was observed.
