The Human IL1R2 CLIA Kit is a cutting-edge assay designed for the precise measurement of IL1R2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL1R2, also known as interleukin-1 receptor type 2, is a key receptor involved in the regulation of inflammation and immune responses. Dysregulation of IL1R2 has been linked to various inflammatory diseases, autoimmune disorders, and cancer, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.
Whether you are studying inflammatory pathways, immune responses, or disease progression, the Human IL1R2 CLIA Kit provides a reliable tool for assessing IL1R2 levels and gaining valuable insights into the underlying mechanisms of your research.
Product Name:
Human IL-1R2 CLIA Kit
SKU:
HUES00087
Target:
Human IL-1R2
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1R2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1R2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1R2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-1R2. You can calculate the concentration of Human IL-1R2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
91-106
87-97
Average (%)
97
97
92
1:4
Range (%)
91-106
89-101
93-109
Average (%)
96
96
100
1:8
Range (%)
98-113
84-99
102-116
Average (%)
105
91
110
1:16
Range (%)
104-115
95-112
85-96
Average (%)
109
103
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.51
100.85
343.93
35.46
100.29
361.66
Standard deviation
3.04
9.33
32.85
4.32
10.38
29.48
C V (%)
8.1
9.25
9.55
12.18
10.35
8.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-1R2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1R2 in samples. No significant cross-reactivity or interference between Human IL-1R2 and analogues was observed.
