The Human IL1RA (Interleukin-1 Receptor Antagonist) CLIA Kit is a powerful tool for accurately measuring IL1RA levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.IL1RA is an important protein that acts as a natural antagonist to interleukin-1, a pro-inflammatory cytokine. By inhibiting the activity of interleukin-1, IL1RA helps regulate the immune response and inflammatory processes in the body.
This makes it a valuable biomarker for studying various inflammatory conditions, autoimmune diseases, and inflammatory-mediated pathologies.With the Human IL1RA CLIA Kit, researchers can accurately measure IL1RA levels in human samples, allowing for a better understanding of its role in health and disease. This kit is an essential tool for studying the intricate immune response and inflammatory pathways, paving the way for potential therapeutic interventions.
Product Name:
Human IL-1ra CLIA Kit
SKU:
HUES00086
Target:
Human IL-1ra
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1ra. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1ra and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1ra, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-1ra. You can calculate the concentration of Human IL-1ra in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-118
86-101
100-112
Average (%)
108
92
105
1:4
Range (%)
89-103
85-101
91-104
Average (%)
94
92
97
1:8
Range (%)
97-111
101-116
92-106
Average (%)
104
109
97
1:16
Range (%)
88-98
92-102
86-100
Average (%)
93
97
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
100-116
108
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.24
143.89
386.21
48.1
134.58
388.18
Standard deviation
5.99
10.86
34.95
5.34
10.58
39.83
C V (%)
12.68
7.55
9.05
11.1
7.86
10.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-1ra concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1ra in samples. No significant cross-reactivity or interference between Human IL-1ra and analogues was observed.
