The Human IL1RA (IL1F3) ELISA Kit is specially designed for the precise measurement of Interleukin 1 Receptor Antagonist (IL1RA) levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent tool for a variety of research applications.IL1RA is a key cytokine that plays a crucial role in regulating the body's inflammatory response by inhibiting the activity of Interleukin-1 (IL-1).
Imbalances in IL1RA levels have been linked to various inflammatory and autoimmune disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.The Human IL1RA (IL1F3) ELISA Kit from AssayGenie provides researchers with a reliable and efficient tool for quantifying IL1RA levels in human samples, aiding in the advancement of scientific understanding and discovery in the field of immunology and inflammatory diseases.
Product Name:
Human IL-1ra/IL-1F3 ELISA Kit
SKU:
HUES01375
Target:
Human IL-1ra/IL-1F3
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1ra/IL-1F3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1ra/IL-1F3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1ra/IL-1F3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1ra/IL-1F3. You can calculate the concentration of Human IL-1ra/IL-1F3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
84-99
90-104
Average (%)
104
91
98
1:4
Range (%)
91-102
80-89
83-96
Average (%)
97
85
88
1:8
Range (%)
91-102
83-95
83-96
Average (%)
96
88
87
1:16
Range (%)
90-104
83-94
87-102
Average (%)
95
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.6
253.23
775.71
104.45
248.93
801.14
Standard deviation
5.12
13.12
41.11
5.89
12.92
43.58
C V (%)
5.3
5.18
5.3
5.64
5.19
5.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-1ra/IL-1F3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Human IL-1ra/IL-1F3 and analogues was observed.
