The Human IL20 CLIA kit (HUES00207) by Assay Genie is a highly sensitive and specific assay designed for the precise measurement of Interleukin-20 levels in human samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing researchers with reliable and reproducible results for a variety of research applications.Interleukin-20 is a key cytokine involved in inflammation and immune responses, playing a crucial role in various diseases such as autoimmune disorders, skin inflammation, and inflammatory bowel disease.
By accurately measuring IL20 levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets.The Assay Genie Human IL20 CLIA kit offers a comprehensive solution for studying the role of Interleukin-20 in health and disease, making it an essential tool for researchers in the fields of immunology, inflammation, and drug discovery.
Product Name:
Human IL-20 CLIA Kit
SKU:
HUES00207
Target:
Human IL-20
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-20. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-20 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-20, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-20. You can calculate the concentration of Human IL-20 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-106
96-109
Average (%)
92
97
103
1:4
Range (%)
99-112
91-105
103-117
Average (%)
105
98
109
1:8
Range (%)
91-105
88-104
88-100
Average (%)
97
95
95
1:16
Range (%)
99-114
86-100
88-101
Average (%)
106
94
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
97
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.37
90.05
341.28
52.61
81.29
316.37
Standard deviation
4.93
7.1
32.97
5.17
7.3
29.96
C V (%)
9.07
7.88
9.66
9.83
8.98
9.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-20 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-20 in samples. No significant cross-reactivity or interference between Human IL-20 and analogues was observed.
