The Human IL2R-Beta CLIA Kit is a high-quality assay designed for the precise measurement of IL2R-Beta levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.IL2R-Beta, also known as interleukin-2 receptor subunit beta, is a critical component in the immune system and plays a key role in regulating immune responses and cell signaling. Abnormal levels of IL2R-Beta have been linked to various autoimmune diseases, inflammatory disorders, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
The Human IL2R-Beta CLIA Kit from Assay Genie provides researchers with a reliable tool for investigating the role of IL2R-Beta in health and disease, offering a precise and efficient way to quantify IL2R-Beta levels in biological samples. Order yours today to advance your research efforts in immunology and beyond.
Product Name:
Human IL-2R beta CLIA Kit
SKU:
HUES00607
Target:
Human IL-2R beta
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Rβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2Rβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2Rβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-2Rβ. You can calculate the concentration of Human IL-2Rβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
89-103
87-99
Average (%)
97
96
93
1:4
Range (%)
102-120
84-98
94-109
Average (%)
109
91
100
1:8
Range (%)
89-101
100-115
103-117
Average (%)
94
109
109
1:16
Range (%)
88-98
89-103
87-98
Average (%)
93
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.99
140.23
470.54
47.59
137.29
435.35
Standard deviation
4.54
16.18
40.84
5.77
10.35
45.97
C V (%)
9.66
11.54
8.68
12.12
7.54
10.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-2Rβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-2Rβ in samples. No significant cross-reactivity or interference between Human IL-2Rβ and analogues was observed.
