The Human IL-4 ELISA Kit is a powerful tool for detecting and measuring interleukin-4 levels in human samples. IL-4 is a key cytokine involved in immune responses, inflammation, and allergic reactions. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in serum, plasma, and cell culture supernatants.IL-4 plays a crucial role in regulating the immune system and can be implicated in various diseases and disorders, including asthma, allergic reactions, and autoimmune diseases.
Studying IL-4 levels can provide valuable insights into the underlying mechanisms of these conditions and aid in the development of targeted therapies.With its advanced technology and easy-to-use format, the Human IL-4 ELISA Kit is an essential tool for researchers and clinicians looking to explore the role of IL-4 in health and disease. Order now and take your research to the next level with this cutting-edge kit from Assay Genie.
Product Name:
Human IL-4 ELISA Kit
SKU:
HUES01387
Target:
Human IL-4
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-4. You can calculate the concentration of Human IL-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-106
98-113
91-105
Average (%)
97
105
96
1:4
Range (%)
96-112
82-95
92-106
Average (%)
104
87
97
1:8
Range (%)
95-110
86-97
93-109
Average (%)
102
91
100
1:16
Range (%)
94-108
88-101
95-112
Average (%)
101
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
93-108
98
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.8
203.6
960.7
107.7
191.5
872.5
Standard deviation
6.2
8.6
33.6
6.6
9.6
26.2
C V (%)
6.03
4.22
3.5
6.13
5.01
3.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
