The Human IL6R CLIA Kit is a cutting-edge assay designed for the precise measurement of the IL6R (Interleukin 6 Receptor) levels in human biological samples such as serum and plasma. With exceptional sensitivity and specificity, this kit provides accurate and reliable results, making it an indispensable tool for researchers in a variety of fields.The IL6R is a critical receptor involved in the inflammatory response and immune regulation, playing a key role in various diseases such as rheumatoid arthritis, inflammatory bowel disease, and certain cancers.
By accurately quantifying IL6R levels, researchers can gain valuable insights into disease progression and potential therapeutic interventions.This CLIA kit from Assay Genie offers unparalleled performance and ease of use, making it suitable for both academic and clinical research settings. Enhance your research capabilities with the Human IL6R CLIA Kit and unlock new possibilities in the study of inflammatory and immune-related disorders.
Product Name:
Human IL-6R CLIA Kit
SKU:
HUES00166
Target:
Human IL-6R
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6R. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-6R. You can calculate the concentration of Human IL-6R in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
100-114
89-100
Average (%)
94
108
95
1:4
Range (%)
97-110
92-110
96-110
Average (%)
102
100
103
1:8
Range (%)
98-110
99-117
89-103
Average (%)
104
107
94
1:16
Range (%)
100-114
86-98
99-114
Average (%)
107
92
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-117
107
EDTA plasma (n=5)
95-107
100
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.15
82.08
311.63
41.4
85.08
287.53
Standard deviation
3.88
7.9
26.24
4.58
7.96
28.9
C V (%)
9.66
9.62
8.42
11.06
9.36
10.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-6R concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-6R in samples. No significant cross-reactivity or interference between Human IL-6R and analogues was observed.
