Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit
The Human IP-10 (CXCL10) CLIA Kit is specifically designed for the precise detection of interferon-gamma-induced protein 10kDa levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for various research applications.IP-10, also known as CXCL10, is a key chemokine involved in immune responses and inflammation, playing a crucial role in recruiting T cells to sites of infection or inflammation.
Elevated levels of IP-10 have been associated with various conditions including autoimmune diseases, viral infections, and certain types of cancer. Therefore, this kit serves as an essential tool for studying the role of IP-10 in these conditions and developing potential therapeutic interventions.Overall, the Human IP-10 (CXCL10) CLIA Kit offers researchers a reliable and efficient method for measuring IP-10 levels in human samples, providing valuable insights into immune responses and inflammatory processes.
Product Name:
Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit
SKU:
HUES00048
Target:
Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IP-10/CXCL10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IP-10/CXCL10. You can calculate the concentration of Human IP-10/CXCL10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
92-105
102-118
Average (%)
98
98
109
1:4
Range (%)
84-96
101-116
98-115
Average (%)
91
109
105
1:8
Range (%)
96-110
87-101
97-111
Average (%)
103
94
104
1:16
Range (%)
103-116
101-117
93-105
Average (%)
109
109
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.6
72.26
192.19
24.27
73.23
175.93
Standard deviation
2.01
6.56
15.34
2.6
8.41
17.43
C V (%)
8.17
9.08
7.98
10.71
11.48
9.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed.
