The Human Keratin 33A (KRT33A) ELISA Kit is a reliable and accurate tool for the quantification of KRT33A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.KRT33A is a key protein that plays a critical role in maintaining the structural integrity of epithelial cells, particularly in the skin and hair.
Abnormalities in KRT33A expression have been linked to various skin disorders and conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With its advanced technology and user-friendly format, the Human Keratin 33A (KRT33A) ELISA Kit is an essential tool for researchers and clinicians seeking to better understand the role of KRT33A in human health and disease.
Product Name:
Human Keratin 33A (KRT33A) ELISA Kit
Product Code:
HUDL01654
Size:
96 Assays
Target:
KRT33A
Synonyms:
KRTHA3A, Ha-3I, Krt1-3, K33A, HHA3-I, HKA3A, Keratin,Hair,Acidic 3A, Hard Keratin Type I 3I, Keratin, type I cuticular Ha3-I
Detection Method:
Sandwich
Range:
78.12-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
