The Human KISS1 (Kisspeptin 1) ELISA Kit is a specialized assay designed for the quantitative analysis of Kisspeptin 1 in human biological samples. Kisspeptin, a neuropeptide encoded by the KISS1 gene, plays a pivotal role in regulating reproductive function by stimulating the secretion of gonadotropin-releasing hormone (GnRH) and subsequent gonadotropin release, impacting fertility, puberty, and other reproductive processes. Our KISS1 ELISA Kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research or clinical studies.
Manufactured under stringent quality control measures, this kit delivers reliable performance and ease of use, making it a reliable choice for investigating Kisspeptin 1 levels in various biological samples. Trust Assay Genie's KISS1 ELISA Kit for precise and dependable quantification of this important biomarker in your experimental endeavors.
Product Name:
Human KISS1 (Kisspeptin 1) ELISA Kit
SKU:
AEES00133
Target:
Human KISS1 (Kisspeptin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KISS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KISS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KISS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KISS1. You can calculate the concentration of Human KISS1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-104
94-103
85-97
Average (%)
101
97
92
1:4
Range (%)
97-105
87-100
93-100
Average (%)
102
95
97
1:8
Range (%)
94-107
94-105
98-107
Average (%)
102
98
103
1:16
Range (%)
95-103
98-112
89-99
Average (%)
99
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-97
92
EDTA plasma (n=5)
87-98
91
Cell culture media (n=5)
88-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.38
219.25
939.15
88.93
224.16
958.91
Standard deviation
5.49
10.02
40.67
5.23
10.98
38.45
C V (%)
5.88
4.57
4.33
5.88
4.9
4.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human KISS1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KISS1 in samples. No significant cross-reactivity or interference between Human KISS1 and analogues was observed.
